0.0 μl Taq buffer, 0.00 μl Taq Polymerase, 0 μl Template cDNA, 0.0 μl dNTP, 0.00 μl each of the designed Reverse and Forward primers were added to the PCR tube. The volume of ddH0O was calculated as 00.00 μl and added to the mixture so that the total volume was 00 μl. These processes were repeated for 0 samples. Then the thermal cycler was set to 00 cycles. For the PCR reaction, initial denaturation was set at 00 °C for 0 minute, for Denaturation for 00 seconds at 00 °C, for Annealing step for 00 seconds at 00 °C, for extension step at 00 °C for 0 minute, and for last extension at 00 °C for 0 minutes. After amplification, Thermo 0 kb DNA Ladder was loaded in the first well and samples was loaded into other wells in the agarose gel. and move at 000 V for 00 min. Lastly, the DNA pieces were imaged under the UV light.