Cutsmart buffer was used for seal 0 restiriction enzyme. One and two coded tubes were arranged with 00 macroliters. Ten macroliters cutsmart reaction buffer were arranged separately in each tube. Each tube was individually arranged with 0 microlitter restiriction enzyme fok0 and then incubated at thirty-seven degrees.And then, the sample loading dye was mixed. Additionally, agorase gel was loaded for agarose gel electrophoresis. The gel was attenuated in the chemiDoc machine. Ethidium bromide was selected from the gel. Bands were visualized with gel.